Mass Barcode Signal Amplification for Multiplex Allergy Diagnosis by MALDI-MS

Mass Barcode Signal Amplification for Multiplex Allergy Diagnosis by MALDI-MS.

A highly sensitive method based on mass-barcoded gold nanoparticles (AuNPs) and immunomagnetic separation has been developed for multiplex allergy diagnosis by MALDI mass spectrometry in a component-resolved manner. Different analytical probes were prepared by coating AuNPs with individual allergenic proteins and mass barcode, represented by polyethylene glycol molecules of various chain length...

Characterization of fengycin homologues produced by Bacillus subtilis K1 by MALDI-TOF tandem mass spectrometry (MALDI TOF MS/MS)

MALDI-TOF MS for the diagnosis of infectious diseases.

BACKGROUND First introduced into clinical microbiology laboratories in Europe, MALDI-TOF MS is being rapidly embraced by laboratories around the globe. Although it has multiple applications, its widespread adoption in clinical microbiology relates to its use as an inexpensive, easy, fast, and accurate method for identification of grown bacteria and fungi based on automated analysis of the mass ...

Identification of wheat endosperm proteins by MALDI mass spectrometry and LC-MS/MS.

The commercial value of the wheat crop is a function of the quality and amount of the storage protein and starch present in the grain, which in turn are influenced by environmental conditions during grain-fill. To understand how environment modifies the size and composition of wheat grains, we set out to identify the key metabolic and regulatory proteins in developing grain. We present results ...

Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA).

This chapter describes a method for the rapid assessment of promoter hypermethylation levels or methylation of imprinted regions in human genomic DNA extracted from various sources using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA). Multiplex ligation-dependent probe amplification (MLPA) is a powerful and easy-to-perform PCR-based technique that can identify g...